Monday, November 10, 2003
Description Genomic DNA Extraction - Quick Protocol Procedure Place one young Arabidopsis leaf into an eppen Homogenise Add 10l of 0.5M NaOH Centrifuge at 15,000 rpm for 30 seconds at room temperature Transfer 5l into an eppen containing 50l of 100mM Tris-HCl This is the DNA stock, store at 30C Use 1l for PCR Recipes Supplies Tips Wang, H., Qi, M. and Cutler, A.J. (1993). A simple method of preparing plant samples for PCR. Nucleic Acids Research. 21(17): 4153-4154 (责任编辑:泉水) |