Tuesday, November 18, 2003 Description Yeast IF without Dehydration Procedure Grow cells at the appropriate temperature to 5x10E6 in 5 mls YPD. Add 0.5mls 37% formaldehyde (best grade) and incubate on the roller at same temp. for 10 min. S...

Tuesday, November 18, 2003 Description Yeast IF with Methanol/Acetone Dehydration Procedure Grow cells at the appropriate temperature to 5x10E6 in 5 mls YPD. Add 0.5mls 37% formaldehyde (best grade) and incubate on the roller at same temp....

Tuesday, November 18, 2003 Description Quantitative Mating Assay (Yeast) Procedure 1. Grow a culture of the strain whose mating efficiency is to be determined to a density of about 1E7 cells/ml in YEPD. Prepare a similar culture of an appr...

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Tuesday, November 18, 2003 Description One of the suggested protocol Procedure Grow up a small (5mL) overnight culture in YEPD (rich glucose media), 30C. Count the cells the next day, and dilute the culture down to 1E6 cells/mL, again into...

Monday, November 10, 2003 Description BAC DNA Fingerprint Analysis Procedure Fingerprinting Protocol (Agarose Gel): Restriction digests consist of: 15.75 ml ddH2O 1 l 10 X buffer B (Boehringer Mannheim) 0.25 l HindIII (40 U/l) 3 l DNA Set...

Friday, November 07, 2003 Description Pollen Development in Anthers of Arabidopsis Procedure . Sow Arabidopsis seeds in Petri dishes on a layer of sterilized superfine grade Perlite pre-wetted with Gamborg's B5 Medium. 2. Incubate in a gro...

Friday, November 07, 2003 Description ARABIDOPSIS TRANSFORMATION Procedure Seed Sterilization. Sterilize WS (Wassilewskija) seed for 10 min in a solution of 50% Chlorox with 0.1% SDS in ependorf tubes. Rinse 3 to 5 times with dH20. Dry tho...

Friday, October 24, 2003 Description Method to transform plasmid DNA into yeast using a small amount of at-the-bench time. Requires 1 overnight to complete. Procedure 1. Take fresh overnight of yeast cells from liquid or media rich plate....