Saturday, May 15, 2004
Description PAH BglII Polymorphism Procedure BglII; LOCATED 5' OF EXON 1 Template: Genomic DNA 100-200 ng/reaction volume of 25 l Primer 1. B2PAH1 5' -GCA GGA AAC TCT CTG ACT TTG G -3' 100 ng/reaction Primer 2. B2PAH2 5' -TGG CAG TTC TGG AGG CCA GA -3' 100 ng/reaction dNTPs: Final Concentration 200 M each Buffer: Final Concentration 1.50 mM MgCl2, 50 mM KCl, 10 mM Tris HCl pH 8.4 Taq DNA Polymerase: 0.5 Units/reaction (Perkin-Elmer Amplitaq DNA polymerase 5 U/l, Cat. No. N808-0145) PCR Cycling Profile: 95C, 5' 95C (30"), 60C (30"), 72C (30") x 30 cycles Product Size: BglII 290 bp ------->209 bp and 81 bp (+/+ individual) Recipes Supplies Tips (责任编辑:泉水) |