C. elegans RNA prep(2)

Supplies

Tips
Precautions against RNase contamination:

Because RNase is incredibly stable and is widely used in labs during DNA preps, precautions must be taken when preparing solutions, plasticware, and glassware for use with RNA to avoid contamination with this enzyme. Glassware (but not the plastic caps!) stir bars, and spatulas for weighing reagents out should be baked overnight in a 180deg. C oven (Horvitz lab: this is in the autoclave room). Plasticware (pipette tips, centrifuge tubes, 10 ml disposable pipettes) should be from an unopened package that is labelled RNase free and then kept in a special drawer after opening. Most solutions, as detailed below, can be treated with diethyl pyrocarbonate (DEPC) to inactivate RNases, and then autoclaved to eliminate the DEPC. DEPC reacts with amino groups, and therefore cannot be used, for example, on Tris buffered solutions. Always wear clean gloves when handling RNase free materials. If at all possible, use unopened clean containers of reagents to make up all solutions, label the bottles "RNase free", and store in a special drawer. When weighing out reagents, clean the balance first and use weigh boats from an unopened container.

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C. elegans RNA prep(2)