PAH PvuII B Polymorphism

Saturday, May 15, 2004

Description
PAH PvuII B Polymorphism

Procedure
PvuII B LOCATED IN INTRON 3

Template: Genomic DNA 100-200 ng/reaction volume of 25 l

Primer 1. PAHPVUb-1 5' GCCTGAAAGCAATACAGAGG 3' 100 ng/reaction
Primer 2. PAHPVUb-2 5' TGAGGTAGGGATGAAAGACC 3' 100 ng/reaction

dNTPs: Final Concentration 200 M each

Buffer: Final Concentration 1.50 mM MgC12, 50 mM KCl, 10 mM Tris HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction
(Perkin-Elmer Amplitaq DNA polymerase 5 U/l, Cat. No. N808-0145)

PCR Cycling Profile: 95C, 5'
95C (30"), 58C (30"), 72C (30") x 30 cycles

Product size: PvuII
489 BP----------> 259 bp and 230 bp (in PvuII site present homozygote)

Recipes

Supplies

Tips

(责任编辑：泉水)

搜索

热门标签:

PAH PvuII B Polymorphism