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Routine TEM Staining Protocol for C(3)

2006-01-22 15:43 bioguider 本站原创 阅读 0

Lead nitrate ------------------------------- 1.33 g

Sodium citrate, dihydrate -------------- 1.76 g (solution becomes cloudy when sodium citrate is added)

1N NaOH ----------------------------------- 5 ml  (solution becomes clear when NaOH is added)

Distilled water ----------------------------- 30 ml

Stir for 10 minutes to dissolve and add additional 15 ml of distilled water. Store solution for 3-6 months at 4 C. Note: the mount of NaOH is very important. Too much will make solution cloudy.

 

Epon 812:

 

Final Volume

EM bed-812

DDSA

NMA

DMP-30 (2%)

20.91 ml

10 ml

4.5 ml

6 ml

0.41 ml

31.37 ml

15 ml

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