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[DNA Purification] Small scale chromosomal DNA isolation from brain tissue 日期：2005-07-18 00:00:00 点击：289 好评：0
Wednesday, June 01, 2005 Description Small scale chromosomal DNA isolation from brain tissue. Suitable for PCR amplification. Procedure 1.Cut +0,25 g brain tissue (best fresh of freshly frozen tissue) in smaller pieces. 2.Add 850ul Lysing...
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[DNA Purification] Small scale chromosomal DNA isolation from hair follicles 日期：2005-07-18 00:00:00 点击：240 好评：0
Wednesday, June 01, 2005 Description Small scale chromosomal DNA isolation from hair follicles. DNA is suitable for PCR amplification techniques. Procedure 1.Take hair samples by pulling from just above the eye (do not cut!! we need the fo...
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[DNA Purification] Elution of DNA fragments from agarose 日期：2005-07-18 00:00:00 点击：276 好评：0
Sunday, June 27, 2004 Description A rapid and very simple method to purify DNA fragments from agarose gel with yield and quality sufficient enough for routine molecular biology work. Procedure 1. Place excised DNA-containing agarose gel sl...
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[DNA Purification] Safeguarding DNA against UV damage during isolation 日期：2005-07-18 00:00:00 点击：246 好评：0
Thursday, June 24, 2004 Description DNA isolated from agarose and acrylamide gels is usually subjected to UV light in the presence of ethidium bromide resulting in crosslinks. The amount of crosslinking is proportional to the time required...
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[DNA Purification] Isolation Total genomic DNA from Peripheral Blood Leukocytes 日期：2005-07-18 00:00:00 点击：225 好评：0
Wednesday, June 23, 2004 Description Following is a modification of Wyman and White PNAS 77:6754-6758 (1980). I've found it a very quick and useful yeilding about 300 ug DNA with a 260/280 ratio of 1.65 - 1.8. Reference: Frank, M. B. Isola...
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[DNA Purification] PLAT Alu Polymorphism 日期：2005-07-18 00:00:00 点击：268 好评：0
Saturday, May 15, 2004 Description PLAT Alu Polymorphism Procedure Template: Genomic DNA 100-200 ng/reaction volume of 25 ul Primer 1: PLAT.A: 5'-GTG AAA AGC AAG GTC TAC CAG-3 100 ng/Rx Primer 2: PLAT.B: 5'-GAC ACC GAG TTC ATC TTG AC-3' 10...
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[DNA Purification] COMT NlaIII Polymorphism 日期：2005-07-18 00:00:00 点击：876 好评：0
Description COMT NlaIII Polymorphism Procedure NlaIII CURRENT PROTOCOL Template: Genomic DNA 300 ng/reaction volume of 25 l Primers: Forward Primer: 5'-GCCCGCCTGCTGTCACC-3', Final Concentration = 200 nM Reverse Primer: 5'-CTGAGGGGCCTGGTGAT...
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[DNA Purification] RET RsaI Polymorphism 日期：2005-07-18 00:00:00 点击：274 好评：0
Saturday, May 15, 2004 Description RET RsaI Polymorphism Procedure Rsa I Polymorphism in exon 15 Template: Genomic DNA 100-200 ng/reaction volume of 25 l. Primer 1. RET15RSA1 5'-GAC TCG TGC TAT TTT TCC TCA3' 100 ng/Reaction Primer 2. RET15...
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[DNA Purification] HOXB4 MspI Polymorphism 日期：2005-07-18 00:00:00 点击：200 好评：0
Saturday, May 15, 2004 Description HOXB4 MspI Polymorphism Procedure MspI in HOXB4; Clone pH 2-1-3 Bg 2 Template: Genomic DNA 100-200 ng/reaction volume of 25 l Primer 1. BG2FIN2 '5-AGG ATG TGT GAG AGG CTG G 100 ng/reaction Primer 2. BG2RI...
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[DNA Purification] D10S94 MspI Polymorphism 日期：2005-07-18 00:00:00 点击：286 好评：0
Saturday, May 15, 2004 Description D10S94 MspI Polymorphism Procedure MspI Template: Genomic DNA 100-200 ng/reaction volume of 25 l Primer 1. S94MSPA 5 -CGA ATT CTT CCT GAA GCC T-3' 90 ng/reaction Primer 2. S94MSPB 5' -TTT GTA ATG GAG TGG...