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[DNA Purification] CHLOROPLAST DNA ISOLATION 日期：2005-07-18 00:00:00 点击：169 好评：0
Friday, November 07, 2003 Description CHLOROPLAST DNA ISOLATION Procedure Grind in blender with 7 vol LL for maize, 10 for sorghum. Use MCE at 350 g/L, and if necessary, 5 ml 1 M DIECA/L. Cut leaves into ca. 1 strips prior to blending. Squ...
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[DNA Purification] NUCLEI ISOLATION 日期：2005-07-18 00:00:00 点击：141 好评：0
Friday, November 07, 2003 Description NUCLEI ISOLATION FROM PLANTS Procedure Harvest, weigh tissue, . Dump into N2 in ice bucket; break up with spatula/scissors (ca. 300 ml N2). Dump into Waring blender; cover with cheesecloth, grind at ma...
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[DNA Purification] ARABIDOPSIS THALIANA DNA ISOLATION PROCEDURE 日期：2005-07-18 00:00:00 点击：135 好评：0
Friday, November 07, 2003 Description Nuclei isolation modified from Hamilton, Kunsch, and Temperli (1972) Anal. Biochem. 49:48-57; Procedure Procedure: (all steps at 0-4C unless indicated) Harvest 100 g tissue (plants can be any age up to...
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[DNA Purification] DNA from plasma 日期：2005-07-18 00:00:00 点击：162 好评：0
Friday, October 24, 2003 Description Sometimes the only material available on a subject is stored plasma or serum samples. The DNA content in such samples is generally low, but sufficient to serve as template for PCR reactions. Many diseas...
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[DNA Purification] Detection of Recombinant Proteins by ELISA 日期：2005-07-18 00:00:00 点击：146 好评：0
Thursday, October 09, 2003 Description Copyright 1993, 1996, and 1997 by Mark Barton Frank, Ph.D. Proper citation for data acquired from this document is: McCubbin, V. and Frank, M. B. Detection of Recombinant Proteins by ELISA. In: Frank,...