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[DNA Sequencing] Method for assaying gliotoxin production in Aspergillus fumi 日期：2005-07-18 00:00:00 点击：184 好评：0
Thursday, November 13, 2003 Description Method for assaying gliotoxin production in Aspergillus fumigatus Procedure Culture and extraction Culture Aspergillus fumigatus on Sabouraud agar plates for 2 days at 37C and harvest the conidia usi...
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[DNA Sequencing] A rapid method for generating gene deletions in Aspergillus 日期：2005-07-18 00:00:00 点击：180 好评：0
Thursday, November 13, 2003 Description A rapid method for generating gene deletions in Aspergillus fumigatus Procedure Step 1: Introduction of target cosmid into KS272(pKOBEG) The E. coli strain KS272(pKOBEG) is transformed with the relev...
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[DNA Sequencing] Random Primed Labeling of DNA 日期：2005-07-18 00:00:00 点击：161 好评：0
Tuesday, October 21, 2003 Description Random Primed Labeling of DNA Procedure 1. Denature 30 to 50 ng of DNA by boiling for 2 min. 2. To a microcentrifuge tube add in this order: ddH2O to give a final volume of 50 ul 10 ul of OLB Denatured...
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[DNA Sequencing] Labeling Oligonucleotides Using T4 Polynucleotide Kinase 日期：2005-07-18 00:00:00 点击：150 好评：0
Tuesday, October 21, 2003 Description Labeling Oligonucleotides Using T4 Polynucleotide Kinase Procedure 1. Add the following to a microcentrifuge tube: 2 l of 10X Kinase Buffer 3 l of -[32P]-ATP (7000 Ci/mmole or 167 Ci/ul) (CAUTION! See...
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[DNA Sequencing] S1 Nuclease Mapping Using Single-Strande 日期：2005-07-18 00:00:00 点击：333 好评：0
Description S1 Nuclease Mapping Using Single-Stranded Radiolabeled Oligonucleotide Probes Procedure 1. Mix in a sterile microcentrifuge tube: 10 ìl of End-labeled, single-stranded, radiolabeled oligonucleotide probe (dilute so that there...
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[DNA Sequencing] DNase I Footprinting 日期：2005-07-18 00:00:00 点击：199 好评：0
Sunday, October 19, 2003 Description An end labeled DNA probe is incubated with a purified DNA-binding factor or with a protein extract. The unprotected DNA is then digested with DNase I such that on average, every DNA molecule is cut once...
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[DNA Sequencing] 5-RACE 日期：2005-07-18 00:00:00 点击：204 好评：0
Wednesday, October 08, 2003 Description Used for cloning of unknown 5-ends (provided internal sequence is available for primer design) Primary Author Larry Simpson ( simpson@hhmi.ucla.edu ) Affiliation University of California Los Angeles...