-
[DNA Sequencing] NGFR TaqI(a) Polymorphism 日期：2005-07-18 00:00:00 点击：293 好评：0
Saturday, May 15, 2004 Description NGFR TaqI(a) Polymorphism Procedure TaqI(a) (7.18 KB DWST OF EXON 6) Template: Genomic DNA 100-200 ng/reaction Primer 1. NGFRTQAF1 5' GAC ACT GGT TAT ACT CTA GTC C 3' 100 ng/reaction Primer 2. NGFRTQAR1 5...
-
[DNA Sequencing] DRD2 TaqI A Polymorphism 日期：2005-07-18 00:00:00 点击：288 好评：0
Description DRD2 TaqI "A" Polymorphism Procedure Taq I "A" Located 10 kb downstream from exon 8 TEMPLATE: Genomic DNA 100-200 ng/reaction volume of 25 l. Primer 1: 5014 MOD: '5- CCT TCC TGA GTG TCA TCA AC 100 ng/reaction Primer 2: TAQACK1:...
-
[DNA Sequencing] RBP3 BglII Polymorphism 日期：2005-07-18 00:00:00 点击：294 好评：0
Saturday, May 15, 2004 Description RBP3 BglII Polymorphism Procedure Bgl II polymorphism located near 5' end of intron 2 Genomic DNA: 100-200 ng/reaction volume of 25 ul Primer 1. RBP3BGL1: 5'-ACC CTG TGA CAC AGC AGA GG-3' 100 ng/Reaction...
-
[DNA Sequencing] DRD2 TaqI B Polymorphism 日期：2005-07-18 00:00:00 点击：307 好评：0
Description DRD2 TaqI "B" Polymorphismq Procedure Taq I "B" Bcl I Located in intron 1, approximately 1 kb upstream from exon 2 (The Taq I "B" and the Bcl I sites are 12 bp apart) Template: Genomic DNA 100-200 ng/ reaction volume of 25 l. P...
-
[DNA Sequencing] RBP3 MspI Polymorphism 日期：2005-07-18 00:00:00 点击：266 好评：0
Saturday, May 15, 2004 Description RBP3 MspI Polymorphism Procedure MspI Polymorphism located near 3' end of intron 1 Template: Genomic DNA 100-200 ng/reaction volume of 25 ul Primer 1. RBP3MSP3: 5'- CTG TAA GAG TTG GAG GT-3' 100 ng/Rx Pri...
-
[DNA Sequencing] D10S94 PvuII Polymorphism 日期：2005-07-18 00:00:00 点击：272 好评：0
Saturday, May 15, 2004 Description D10S94 PvuII Polymorphism Procedure Pvu II Template: Genomic DNA 100-200 ng/reaction volume of 25 l Primer 1. S94PVUA 5 -AGCTCACCCAGATCCTAA -3' 90 ng/reaction Primer 2. S94PVUB 5' -CCTCCCTTCAGTAGACAC -3'...
-
[DNA Sequencing] ACADM TaqI Polymorphism 日期：2005-07-18 00:00:00 点击：274 好评：0
Saturday, May 15, 2004 Description ACADM TaqI Polymorphism Procedure TaqI site (located in intron 7) Template: Genomic DNA 100-200 ng/reaction volume of 25 l Primer 1. ACADMTAQI: 5'-GAT CCA GTG ACT CAG AAG-3' 100 ng/reaction Primer 2. ACAD...
-
[DNA Sequencing] DM1 Alu Polymorphism 日期：2005-07-18 00:00:00 点击：284 好评：0
Saturday, May 15, 2004 Description DM1 Alu Polymorphism Procedure Template: Genomic DNA 100-200 ng/reaction volume of 25 ul Primer 1: 405: 5'-CTG TAT ACT CAG CTA CTA GGG T-3' 50 ng/rx Primer 2: 491: 5'-AAA TAG GCT GGA CCG CGG-3' 50 ng/rx P...
-
[DNA Sequencing] For Double-stranded reactions: Sequenase[TM] catalyzed seque 日期：2005-07-18 00:00:00 点击：273 好评：0
Thursday, November 20, 2003 Description For Double-stranded reactions Procedure 1. Add the following to a 1.5 ml microcentrifuge tube: 5 ulds DNA (5 ug) 4 ul1 N NaOH 3 ulddH2O 12 ul 2. Incubate the reaction at 65-70degC for 5 minutes, and...
-
[DNA Sequencing] Taq-polymerase catalyzed cycle sequencing using fluorescent- 日期：2005-07-18 00:00:00 点击：192 好评：0
Thursday, November 20, 2003 Description One of the major problems in DNA cycle sequencing is that when fluorescent primers are used the reaction conditions are such that the nested fragment set distribution is highly dependent upon the tem...