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[DNA Library] -Galactosidase Staining of Eukaryotic Cells in vitro 日期：2005-07-18 00:00:00 点击：169 好评：0
Saturday, October 18, 2003 Description Cells that were previously transfected with a lac Z construct can be stained for -Galactosidase activity. Procedure 1. Wash the cells 2 to 3 times in 2 ml of PBS solution (see Hint #2 and Hint #3). 2....
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[DNA Library] High Efficiency Transformation of Yeast Cells 日期：2005-07-18 00:00:00 点击：134 好评：0
Saturday, October 18, 2003 Description This protocol uses a lithium acetate/PEG treatment to transform yeast with DNA. Plasmid DNA or linear DNA may be used Procedure 1. Use cells from an overnight yeast culture to inoculate 300 ml of liqu...
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[DNA Library] Transfection of J774 using FuGENE 6 reagent 日期：2005-07-18 00:00:00 点击：178 好评：0
Thursday, October 16, 2003 Description A method that worked for me, pretty close to the commercial description Procedure 1 - Preparation of cells for transfection Day 1: Plate the cells one-day before the transfection experiment. The appro...
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[DNA Library] Plasmid DNA isolation (maxi-prep) 日期：2005-07-18 00:00:00 点击：215 好评：0
Thursday, October 16, 2003 Description Protocol for large scale Plasmid DNA isolation Procedure Y To be read as micro (mu) 1.5ml of LB medium containing 50Yg/Yl ampicillin [Sigma A-0166] was innoculated with a single bacterial colony. The...
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[DNA Library] Competent cell preparation 日期：2005-07-18 00:00:00 点击：255 好评：0
Wednesday, October 08, 2003 Description For preparation of competent bacterial cells (14), a glycerol cell culture stock of the respective E. coli strain is thawed and added to 50 ml of liquid media. This culture then is preincubated at 37...
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[DNA Library] DNA ligation 日期：2005-07-18 00:00:00 点击：704 好评：0
Wednesday, October 08, 2003 Description DNA ligations are performed by incubating DNA fragments with appropriately linearized cloning vector in the presence of buffer, rATP, and T4 DNA ligase (1,2). For random shotgun cloning, sonicated or...
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[DNA Sequencing] NGFR TaqI(a) Polymorphism 日期：2005-07-18 00:00:00 点击：293 好评：0
Saturday, May 15, 2004 Description NGFR TaqI(a) Polymorphism Procedure TaqI(a) (7.18 KB DWST OF EXON 6) Template: Genomic DNA 100-200 ng/reaction Primer 1. NGFRTQAF1 5' GAC ACT GGT TAT ACT CTA GTC C 3' 100 ng/reaction Primer 2. NGFRTQAR1 5...
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[DNA Sequencing] DRD2 TaqI A Polymorphism 日期：2005-07-18 00:00:00 点击：288 好评：0
Description DRD2 TaqI "A" Polymorphism Procedure Taq I "A" Located 10 kb downstream from exon 8 TEMPLATE: Genomic DNA 100-200 ng/reaction volume of 25 l. Primer 1: 5014 MOD: '5- CCT TCC TGA GTG TCA TCA AC 100 ng/reaction Primer 2: TAQACK1:...
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[DNA Sequencing] RBP3 BglII Polymorphism 日期：2005-07-18 00:00:00 点击：294 好评：0
Saturday, May 15, 2004 Description RBP3 BglII Polymorphism Procedure Bgl II polymorphism located near 5' end of intron 2 Genomic DNA: 100-200 ng/reaction volume of 25 ul Primer 1. RBP3BGL1: 5'-ACC CTG TGA CAC AGC AGA GG-3' 100 ng/Reaction...
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[DNA Sequencing] DRD2 TaqI B Polymorphism 日期：2005-07-18 00:00:00 点击：307 好评：0
Description DRD2 TaqI "B" Polymorphismq Procedure Taq I "B" Bcl I Located in intron 1, approximately 1 kb upstream from exon 2 (The Taq I "B" and the Bcl I sites are 12 bp apart) Template: Genomic DNA 100-200 ng/ reaction volume of 25 l. P...