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[DNA Sequencing] S1 Nuclease Mapping Using Single-Strande 日期：2005-07-18 00:00:00 点击：333 好评：0
Description S1 Nuclease Mapping Using Single-Stranded Radiolabeled Oligonucleotide Probes Procedure 1. Mix in a sterile microcentrifuge tube: 10 ìl of End-labeled, single-stranded, radiolabeled oligonucleotide probe (dilute so that there...
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[DNA Sequencing] DNase I Footprinting 日期：2005-07-18 00:00:00 点击：199 好评：0
Sunday, October 19, 2003 Description An end labeled DNA probe is incubated with a purified DNA-binding factor or with a protein extract. The unprotected DNA is then digested with DNase I such that on average, every DNA molecule is cut once...
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[DNA Sequencing] 5-RACE 日期：2005-07-18 00:00:00 点击：204 好评：0
Wednesday, October 08, 2003 Description Used for cloning of unknown 5-ends (provided internal sequence is available for primer design) Primary Author Larry Simpson ( simpson@hhmi.ucla.edu ) Affiliation University of California Los Angeles...
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[DNA Purification] Small scale chromosomal DNA isolation from brain tissue 日期：2005-07-18 00:00:00 点击：289 好评：0
Wednesday, June 01, 2005 Description Small scale chromosomal DNA isolation from brain tissue. Suitable for PCR amplification. Procedure 1.Cut +0,25 g brain tissue (best fresh of freshly frozen tissue) in smaller pieces. 2.Add 850ul Lysing...
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[DNA Purification] Small scale chromosomal DNA isolation from hair follicles 日期：2005-07-18 00:00:00 点击：240 好评：0
Wednesday, June 01, 2005 Description Small scale chromosomal DNA isolation from hair follicles. DNA is suitable for PCR amplification techniques. Procedure 1.Take hair samples by pulling from just above the eye (do not cut!! we need the fo...
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[DNA Purification] Elution of DNA fragments from agarose 日期：2005-07-18 00:00:00 点击：276 好评：0
Sunday, June 27, 2004 Description A rapid and very simple method to purify DNA fragments from agarose gel with yield and quality sufficient enough for routine molecular biology work. Procedure 1. Place excised DNA-containing agarose gel sl...
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[DNA Purification] Safeguarding DNA against UV damage during isolation 日期：2005-07-18 00:00:00 点击：246 好评：0
Thursday, June 24, 2004 Description DNA isolated from agarose and acrylamide gels is usually subjected to UV light in the presence of ethidium bromide resulting in crosslinks. The amount of crosslinking is proportional to the time required...
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[DNA Purification] Isolation Total genomic DNA from Peripheral Blood Leukocytes 日期：2005-07-18 00:00:00 点击：225 好评：0
Wednesday, June 23, 2004 Description Following is a modification of Wyman and White PNAS 77:6754-6758 (1980). I've found it a very quick and useful yeilding about 300 ug DNA with a 260/280 ratio of 1.65 - 1.8. Reference: Frank, M. B. Isola...
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[DNA Purification] PLAT Alu Polymorphism 日期：2005-07-18 00:00:00 点击：268 好评：0
Saturday, May 15, 2004 Description PLAT Alu Polymorphism Procedure Template: Genomic DNA 100-200 ng/reaction volume of 25 ul Primer 1: PLAT.A: 5'-GTG AAA AGC AAG GTC TAC CAG-3 100 ng/Rx Primer 2: PLAT.B: 5'-GAC ACC GAG TTC ATC TTG AC-3' 10...
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[DNA Purification] COMT NlaIII Polymorphism 日期：2005-07-18 00:00:00 点击：876 好评：0
Description COMT NlaIII Polymorphism Procedure NlaIII CURRENT PROTOCOL Template: Genomic DNA 300 ng/reaction volume of 25 l Primers: Forward Primer: 5'-GCCCGCCTGCTGTCACC-3', Final Concentration = 200 nM Reverse Primer: 5'-CTGAGGGGCCTGGTGAT...