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[DNA Microarray] DNA Band Shift Assay 日期：2005-07-18 00:00:00 点击：263 好评：0
Sunday, October 19, 2003 Description The protocol is designed to detect protein-DNA complexes. The DNA is radiolabeled then mixed with the protein. The protein binding can be detected because the protein-DNA complexes have a slower mobilit...
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[DNA Microarray] Terminal deoxynucleotidyl Transferase-mediated dUTP nick end 日期：2005-07-18 00:00:00 点击：256 好评：0
Friday, October 17, 2003 Description Terminal deoxynucleotidyl Transferase-mediated dUTP nick end labeling (TUNEL) is an in situ method for detecting the 3'-OH ends of DNA exposed during the internucleosomal cleavage that occurs during apo...
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[DNA Microarray] Western Blot Protocol 日期：2005-07-18 00:00:00 点击：197 好评：0
Thursday, October 09, 2003 Description In a Western blot, proteins that are separated on polyacrylamide gels on the basis of size are transferred to a membrane for detection with antibodies. Copyright 1993, 1996, and 1997 by Mark Barton Fr...
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[Cell Culture] Differentiation of 3T3-L1 fibroblasts to adipocytes 日期：2006-07-24 14:27:29 点击：389 好评：2
Tuesday, June 14, 2005 Description We maintain the stock of 3T3-L1 cells in 150 mm tissue culture dishes in DMEM (Gibco catalog no. 11995-065) supplemented with 10% Fetal Bovine Serum, 100 units/ml of Penicillin and 100 g/mL of Streptomyci...
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[Cell Culture] Dorsal Root Ganglion Neuron Culture from Adult Rats 日期：2006-07-24 14:27:29 点击：377 好评：0
Sunday, June 12, 2005 Description Procedure for culturing and maintaining Dorsal Root Ganglion (DRG) neurons from adult rats. Procedure Preparing Culture Surface -Collagen coat cover slips (if using) or cell culture dishes: evenly spread 2...
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[Cell Culture] Primary neuron viability 日期：2006-07-24 14:27:28 点击：318 好评：0
Wednesday, June 01, 2005 Description Fluorescence based asssay for establishing neuronal viability in cell culture monolayers. Procedure Monolayer cultures of rat embryonic cortical neurons were prepared and plated at a density of 2-5 x 10...
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[Cell Culture] Protein extraction for SDS-PAGE from human monocytes 日期：2006-07-24 14:27:28 点击：318 好评：0
Wednesday, May 18, 2005 Description Protocol for rapid extraction of undegraded proteins from cultured human monocytes for the purpose of immunoblotting Procedure Isolate PBMCs from Buffy Coats as usual, and select CD14+ monocytes using an...
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[Cell Culture] Cell Clonogenic Survival Assay 日期：2006-07-24 14:27:27 点击：351 好评：0
Wednesday, May 04, 2005 Description Allows one to test the capability of adherent cells to survive and replicate following insult with chemicals or radiation. Procedure 1. Grow the cells in regular media to 70% confluence and treat the cel...
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[Cell Culture] Bulk synthetic RNA preparation 日期：2006-07-24 14:27:27 点击：240 好评：0
Saturday, April 30, 2005 Description A method to prepare large ammounts or synthetic RNA using T7, T3 or SP6 RNA polymerases. Procedure Template Preparation and RNA Transcription PCR generated templates or 2 x Cesium Cl2 banded plasmid DNA...
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[Cell Culture] How to freeze mamalian cells 日期：2006-07-24 14:27:26 点击：309 好评：0
Tuesday, June 29, 2004 Description This protocol describes how to freeze tissue culture cells (mamalian) in a way that they will be viable when thawed. Procedure 1.) Aspirate media 2.) Wash cells with PBS 3.) Apply trypsin/EDTA (1ml for 15...