Monday, November 10, 2003 Description Plant Crosse Protocol Procedure weed out the plant pot, leave only about 3 to 8 tall and vigorous plants (in a regular 5 cm pot) on an individual inflorescence, cut off all the opened flowers (older th...

Friday, November 07, 2003 Description Cell Suspension Culture of Arabidopsis Procedure 1. Immerse Arabidopsis seeds in 10% Household Bleach for 20 min. 2. Rinse the seeds twice with 500 ml of sterile ddH2O and allow them to dry overnight i...

Friday, November 07, 2003 Description Green lab protocol for vacuum infiltration transformation of Arabidopsis Procedure 1. Sow seeds of ecotype Columbia in lightweight plastic pots prepared in the following way: mound Arabidopsis soil mix...

Friday, November 07, 2003 Description MITOCHONDRIAL DNA ISOLATION Procedure Grind in mortar and pestle or Waring blender with 5-7 volumes buffer A per g tissue. Use MCE at 350 l/L, and if necessary, with 5 ml 1 M DIECA/L. Squeeze through c...

Monday, October 27, 2003 Description Preparing Chromosome Spreads Procedure 1. Expose an exponentially-growing culture of cells to Colcemid (CAUTION! see Hint #1) in culture medium to arrest cells in the mitotic phase of the cell cycle (se...

Monday, October 27, 2003 Description Metaphase Block for Cell Synchronization Procedure 1. Remove the medium from an exponentially-growing cell culture and rinse it with 10 ml of PBS. (See Hint #1) 2. Replace the PBS with Complete Medium w...

Monday, October 27, 2003 Description G2 Phase Block with Hoechst 33342 for Cell Synchronization Procedure 1. Presynchronize cells at G0 by serum starvation. Add Low Serum Medium to cells at 70% confluency and allow cells to grow in this me...

Monday, October 27, 2003 Description G1/S Phase Block for Cell Synchronization Procedure 1. Presynchronize cells at G0 by serum starvation. Add Low Serum Medium to cells at 70% confluency and allow cells to grow in this medium for 2 days....

Tuesday, October 21, 2003 Description This protocol describes a method for studying chemotaxis (migration towards a concentration gradient of chemoattractant) of leukocytes (neutrophils, monocytes and lymphocytes) or other migratory cells....

Tuesday, October 21, 2003 Description This protocol describes a procedure for isolating human peripheral blood mononuclear cells (lymphocytes and monocytes) from a Buffy Coat (obtained from a blood bank). Platelets, red blood cells, and ne...