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  • [Cell Culture] HUMAN SOLUBLE INTERLEUKIN-2 RECEPTOR 日期:2006-07-24 14:27:08 点击:148 好评:0

    Wednesday, May 05, 2004 Description IL-2 receptor alpha chain is one of the key components of the lymphoid cell receptor complex. Interleukin-2(IL-2), plays a pivotal role in initiating and potentiating the immune response. It provides a c...

  • [Cell Culture] Pediatric Virus-specific Bulk Stimulation Cytotoxic T Lympho 日期:2006-07-24 14:27:07 点击:129 好评:0

    Wednesday, May 05, 2004 Description This protocol is designed to increase the sensitivity of detection of HIV-1 specific CTL by the antigen-stimulation of PBMC. Stimulator cells are prepared by infecting autologous B-lymphoblastoid cell li...

  • [Cell Culture] Antibody Neutralization of IFNg-Protection from Viral Infect 日期:2006-07-24 14:27:06 点击:137 好评:0

    Monday, May 03, 2004 Description Antibody Neutralization of IFNg-Protection from Viral Infection of L929 and A549 Cell Lines Procedure Materials L929 mouse fibroblast line (ATCC Cat. No. CCL-1) or A549 human lung carcinoma (ATCC Cat. No. C...

  • [Cell Culture] Antibody Neutralization of TNFa-Induced Killing of L929 Cell 日期:2006-07-24 14:27:06 点击:162 好评:0

    Monday, May 03, 2004 Description Antibody Neutralization of TNFa-Induced Killing of L929 Cell Line Procedure Materials L929 mouse fibroblast line (ATCC Cat. No. CCL-1) Culture Medium (RPMI supplemented with 10% FBS) Assay Medium (RPMI supp...

  • [Cell Culture] Antibody Neutralization of Cytokine-induced Proliferation of 日期:2006-07-24 14:27:05 点击:119 好评:0

    Monday, May 03, 2004 Description Antibody Neutralization of Cytokine-induced Proliferation of Indicator Cell Lines Procedure Materials Indicator cell line (see Quick Guide Chart for a given cytokine) Culture Medium (RPMI supplemented with...

  • [Cell Culture] Cytokine ELISPOT Protocol 日期:2006-07-24 14:27:05 点击:119 好评:0

    Monday, May 03, 2004 Description ELISPOT (Enzyme-linked ImmunoSPOT) technique was originally used to enumerate antibody secreting B cells. In the current technique, cells are deposited onto a membrane coated with one antibody specific for...

  • [Cell Culture] IFN-gamma Protection from Viral Infection of Cell Lines 日期:2006-07-24 14:27:04 点击:92 好评:0

    Monday, May 03, 2004 Description IFN-gamma Protection from Viral Infection of Cell Lines Procedure Materials L929 mouse fibroblast line (ATCC Cat. No. CCL-1) or A549 human lung carcinoma (ATCC Cat. No. CCL-185) Culture Medium (RPMI supplem...

  • [Cell Culture] TNF-alpha-Induced Killing of L929 Cell Line 日期:2006-07-24 14:27:04 点击:142 好评:0

    Monday, May 03, 2004 Description TNF-alpha-Induced Killing of L929 Cell Line Procedure Materials L929 mouse fibroblast line (ATCC Cat. No. CCL-1) Culture Medium (RPMI supplemented with 10% FBS) Assay Medium (RPMI supplemented with 2% FBS)...

  • [Cell Culture] Cytokine-Induced Proliferation of Indicator Cell Lines 日期:2006-07-24 14:27:03 点击:80 好评:0

    Monday, May 03, 2004 Description Cytokine-Induced Proliferation of Indicator Cell Lines Procedure Materials Indicator cell line (see Quick Guide Chart for a given cytokine) Culture Medium (RPMI-1640 supplemented with 10% FBS) 96-well flat-...

  • [Cell Culture] PEROXIDASE-ANTI-PEROXIDASE TECHNIQUE FOR THE IDENTIFICATION 日期:2006-07-24 14:27:03 点击:65 好评:0

    Monday, May 03, 2004 Description PEROXIDASE-ANTI-PEROXIDASE TECHNIQUE FOR THE IDENTIFICATION OF ANTIGENS IN PARAFFIN WAX SECTIONS Procedure Dewax sections and transfer to 100% alcohol. Block endogenous peroxidase with 0.5% hydrogen peroxid...

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