Friday, November 21, 2003 Description Protocol for Lyophilization of bacteria Procedure Inoculate 200 ml L-broth supplemented with appropriate antibiotics with the bacteria to be lyophilized. Incubate the culture at 37C with vigorous shaki...

Friday, November 21, 2003 Description This protocol describes a simple procedure for isolating chloroplasts from spinach leaves. Procedure 1. Prepare an ice bath and pre-cool all glassware to be used (including a mortar and pestle). 2. Sel...

Friday, November 21, 2003 Description The first print of the stem will contain a lot of chlorophyll but the second or third print should reveal the position of antigens. This method transfers soluble molecules of all sorts to the membrane....

Friday, November 21, 2003 Description Thylakoid membranes are prepared Procedure Thylakoid membranes are prepared by homogenizing 40 g of fresh, deveined, market spinach (Spinacia oleracea) in 200 mL of Grinding Buffer with a chilled kitch...

Wednesday, November 19, 2003 Description Although this method was developed for use in screening clones constructed in the pRSET family of vectors (Invitrogen), it can just as easily be used with any vector system with the correct set of p...

Tuesday, November 18, 2003 Description Pheromone Halo Assay Procedure -Use sterile technique and sterile solutions throughout this method.- 1. Grow a starter culture at 30 C with shaking (250 rpm) until it reaches saturation. ***It is impo...

Tuesday, November 18, 2003 Description Yeast Prep for FACS Procedure 1. Spin down 1E7 cells in a microfuge tube for 1 minute. 2. Resuspend pellet in 1 ml of 70% EtOH. Fix for at least 60 minutes at room temperature (or up to several days a...

Thursday, November 13, 2003 Description Platelia Aspergillus (Biorad) is a 1-stage immunoenzymatic sandwich microplate kit, for the detection of antigen galactomannan Procedure In a laminar flow safety cabinet (class II), fine cut the samp...

Thursday, November 13, 2003 Description In vitro growth of seedlings Procedure sterilisation of seeds: rinse with 70% EtOH for 30 sec put in 1% bleach (sodium hypochlorite, supplemented by a few drops of Tween-20) for 5 min wash 2-4x with...

Monday, November 10, 2003 Description Protocol for determination of seed moisture content Procedure General Considerations 1. The total weight of seeds used for a moisture content determination should be sufficient to make the test accurat...