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  • [Cell Culture] PP2C assay 日期:2006-07-24 14:26:51 点击:105 好评:0

    Thursday, December 04, 2003 Description PP2C assay Procedure 1. Phosphorylation of casein Casein kinaseI (NEB), 1000U/ul Sigma casein C-4765, 5% solution (56mg/ml), dilute to 10mg/ml 500ul reaction, 30oC, 4h 50 mM Tris-HCl, pH 7.5 10 mM Mg...

  • [Cell Culture] Rice Bombardment 日期:2006-07-24 14:26:50 点击:104 好评:0

    Description Rice Bombardment Procedure DAY 1 1. Filtering Rice cells with 640 m mesh to get small size cells. 2. Discarding the sup 3. Mountaining #4 whatman paper on the N6D 0.5 media 4. Spread 250mg cell on the paper circulary, 1-layerd....

  • [Cell Culture] Agrobacterium-mediated Tobacco Transformation 日期:2006-07-24 14:26:50 点击:71 好评:0

    Description Agrobacterium-mediated Tobacco Transformation Procedure 1. Agrobacterium culture and Harvest: Agrobacterium overnight culture in YEP cfg 5000pm, washing in MS, dilution (1/10 of original volum) with MS 2. Tobacco leaf steriliza...

  • [Cell Culture] ARABIDOPSIS ROOT TRANSFORMATION ASSAYS 日期:2006-07-24 14:26:49 点击:69 好评:0

    Thursday, December 04, 2003 Description ARABIDOPSIS ROOT TRANSFORMATION ASSAYS Procedure SEED STERILIZATION 1. Sterilize Arabidopsis seeds for 10 min in a solution of 50% bleach plus 0.1% SDS. 2. Rinse 5 times with sterile dH2O. 3. Place 5...

  • [Cell Culture] Electrophoretic Mobility Shift Assay (EMSA) for the detectio 日期:2006-07-24 14:26:48 点击:141 好评:0

    Monday, November 24, 2003 Description Electrophoretic Mobility Shift Assay (EMSA) for the detection of nuclear NF-kB Procedure Nuclear extracts For the preparation of nuclear extracts the cells are gently lysed in a NP-40 containing sucros...

  • [Cell Culture] Culture of Human Prostatic Carcinoma Cell Lines 日期:2006-07-24 14:26:47 点击:113 好评:0

    Monday, November 24, 2003 Description Culture of Human Prostatic Carcinoma Cell Lines Procedure Growing and splitting the cells The human prostatic carcinoma cell lines PC3, DU145, JCA1, ALVA31, and LNCaP can be cultured in standard RPMI c...

  • [Cell Culture] Gentle lysis of cells in sucrose-containing Mito-Buffer 日期:2006-07-24 14:26:46 点击:134 好评:0

    Monday, November 24, 2003 Description Lysis in the sucrose-containing Buffer (Mito-Buffer) is supposed to prevent accidential disrupture of the mitochondria to prevent the leakage of mitochondrial proteins (such as cytochrome c) into the c...

  • [Cell Culture] Acridine Orange Stain 日期:2006-07-24 14:26:46 点击:124 好评:0

    Monday, November 24, 2003 Description Acridine orange is a fluorescent dye which will bind to the nucleic acid of bacteria and other cells. It is recommended for use for the detection of microorganisms in direct smears. It is useful for th...

  • [Cell Culture] Leishmans stain for blood cells and parasites 日期:2006-07-24 14:26:45 点击:131 好评:0

    Monday, November 24, 2003 Description Staining protocol Procedure Prepare cytospin slide or smear of sample. Either spray the wet preparation with Smear Fix, leave for 1-2 mins, wash off the fixative with distilled water and drain.... Or l...

  • [Cell Culture] Giemsa for blood cells and parasites 日期:2006-07-24 14:26:45 点击:131 好评:0

    Monday, November 24, 2003 Description Staining protocol Procedure For smears or cytospin preparations: Fix prepared film in methanol - 3 to 5 minutes. Stain in a mixture of 1ml of Giemsa stock plus 2mls of pH6.4 buffer and 47mls of distill...

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