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[DNA Microarray] Preparation of Fluorescent DNA Probe from HUMAN mRNA or Tota 日期：2005-07-18 00:00:00 点击：205 好评：0
Friday, November 21, 2003 Description Preparation of Fluorescent DNA Probe from HUMAN mRNA or Total RNA using Direct Incorporation Procedure I. Preparing fluoresenctly labeled cDNA (probe): To anneal primer, mix 2ug of mRNA or 50-100mg tot...
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[DNA Microarray] Hybridization of Arrays 日期：2005-07-18 00:00:00 点击：207 好评：0
Friday, November 21, 2003 Description Hybridization of Arrays Procedure Directions for fluorescent DNA probes: 1. Final probe volume should be 12-15uL, at 4X SSC, containing competitor DNA etc. as required (e.g., polydA, CoT1 DNA for a hum...
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[DNA Microarray] Post-Processing of Arrays 日期：2005-07-18 00:00:00 点击：275 好评：0
Friday, November 21, 2003 Description Post-Processing of Arrays Procedure Mark boundaries of array on back of slide using diamond scriber. Array will become invisible after post-processing. Fill bottom of humid chamber with 100 ml 1X SSC....
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[DNA Microarray] Protocol for Reverse Transcription and Amino-allyl Coupling 日期：2005-07-18 00:00:00 点击：217 好评：0
Friday, November 21, 2003 Description Protocol for Reverse Transcription and Amino-allyl Coupling of RNA Procedure A. RT Reaction 1. To anneal primer, mix 1-2 mg mRNA with 5 ug of anchored oligo-dT [(dT)20-VN] (Operon, HPLC purified) in a...
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[DNA Microarray] Amino-allyl Reverse Transcription 日期：2005-07-18 00:00:00 点击：198 好评：0
Friday, November 21, 2003 Description Resuspend the contents of one pack of the monofunctional NHS-ester Cy3 or Cy5 dye (mono-functional Cy3 or Cy5 reactive pack, Amersham catalog PA 23001 and PA 25001) in 72 ul DMSO. Aliquot 16 x 4.5 ul a...
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[DNA Microarray] Poly-l-Lysine coating of slides For Microarray 日期：2005-07-18 00:00:00 点击：266 好评：0
Friday, November 21, 2003 Description Poly-l-Lysine coating of slides Procedure 1. Place slides in slide racks. Place racks in chambers. 2. Prepare CLEANING SOLUTION: Dissolve 70 g NaOH in 280 mL ddH2O. Add 420 mL 95% ethanol. Total volume...
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[DNA Microarray] Fluorescent Probe Preparation from RNA&n 日期：2005-07-18 00:00:00 点击：202 好评：0
Description The following protocol describes a process for making Cy3- and Cy5-labeled fluorescent DNA probes from RNA samples, using reverse transcription and an amino-allyl modified nucleotide for esterification of the Cyanine dyes. It i...
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[DNA Microarray] Uracil Interference Assay 日期：2005-07-18 00:00:00 点击：176 好评：0
Sunday, October 19, 2003 Description Uracil Interference Assay Procedure 1. Label the 5' end of the oligonucleotide primers with [32P] (CAUTION See Hint #1) and T4 Polynucleotide Kinase. The oligonucleotides should flank the protein-bindin...
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[DNA Microarray] DNA Band Shift Assay 日期：2005-07-18 00:00:00 点击：263 好评：0
Sunday, October 19, 2003 Description The protocol is designed to detect protein-DNA complexes. The DNA is radiolabeled then mixed with the protein. The protein binding can be detected because the protein-DNA complexes have a slower mobilit...
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[DNA Microarray] Terminal deoxynucleotidyl Transferase-mediated dUTP nick end 日期：2005-07-18 00:00:00 点击：256 好评：0
Friday, October 17, 2003 Description Terminal deoxynucleotidyl Transferase-mediated dUTP nick end labeling (TUNEL) is an in situ method for detecting the 3'-OH ends of DNA exposed during the internucleosomal cleavage that occurs during apo...