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[DNA Purification] D10S94 MspI Polymorphism 日期：2005-07-18 00:00:00 点击：286 好评：0
Saturday, May 15, 2004 Description D10S94 MspI Polymorphism Procedure MspI Template: Genomic DNA 100-200 ng/reaction volume of 25 l Primer 1. S94MSPA 5 -CGA ATT CTT CCT GAA GCC T-3' 90 ng/reaction Primer 2. S94MSPB 5' -TTT GTA ATG GAG TGG...
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[DNA Purification] Hybridization FOR CGH 日期：2005-07-18 00:00:00 点击：221 好评：0
Saturday, May 15, 2004 Description Hybridization FOR CGH Procedure . precipitate DNA and dilute in hybridization solution: 10 l tumor-DNA (Biotin-labeled) 10 l Normal-DNA (Digoxigenin-labeled) 30 l human Cot1-DNA 1 l salmon sperm-DNA 5,1 l...
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[DNA Purification] DNA detection CGH 日期：2005-07-18 00:00:00 点击：208 好评：0
Saturday, May 15, 2004 Description DNA detection CGH Procedure Wash slides 3 x 3 min at 37C in FA / 2x SSC ( cuvette). Wash slides 3 x 2 min at 60C in 0.1x SSC ( cuvette). Keep slides in 4x SSC / 0.1% Tween20 at 37C ( cuvette) unt...
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[DNA Purification] DNA preparation by cryotom tissue dissection 日期：2005-07-18 00:00:00 点击：247 好评：0
Saturday, May 15, 2004 Description DNA preparation by cryotom tissue dissection Procedure Preparations/Materials: Cool cryostat down to -20 to -30C about 3 hours prior to dissection Label eppendorf tube (2 ml, e.g. Safe Lock) and microscop...
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[DNA Purification] C. elegans RNA prep 日期：2005-07-18 00:00:00 点击：133 好评：0
Monday, May 03, 2004 Description C. elegans RNA preps that have been widely used previously involved fairly slow lysis of the worms, potentially leading to degradation of the RNA. In addition, they failed to separate RNA from genomic DNA,...
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[DNA Purification] Maxi-plasmid DNA Prep 日期：2005-07-18 00:00:00 点击：340 好评：0
Thursday, December 04, 2003 Description Maxi-plasmid DNA Prep Procedure 1) Use fresh culture left from mini-prep (0.5 ml) or use freshly transformed cells from a plate (inoculate with a 10 ul loop of cells) 2) Use terrific broth (Gibco): 2...
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[DNA Purification] 10-min plasmid DNA miniprep method 日期：2005-07-18 00:00:00 点击：274 好评：0
Thursday, December 04, 2003 Description A 10-min plasmid DNA miniprep method Procedure 1. Spin down 1.5 ml of E. coli cells in a microfuge (13 Krpm, room temp., 30 to 45 sec) and remove medium 2. Add 50 ml of TEN (10 mM Tris-HCl, pH 8.0, 1...
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[DNA Purification] Fluorescence microscopy of DAPI stained cells 日期：2005-07-18 00:00:00 点击：267 好评：0
Monday, November 24, 2003 Description 4'-6-Diamidino-2-phenylindole (DAPI) is known to form fluorescent complexes with natural double-stranded DNA, showing a fluorescence specificity for AT, AU and IC clusters. Because of this property DAP...
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[DNA Purification] DNA laddering assay for treated cells 日期：2005-07-18 00:00:00 点击：236 好评：0
Monday, November 24, 2003 Description The cells are prefixed in 70% ethanol and, if wished, can be stored in this way for several weeks before analysis. After fixing, the cells are extracted with 0.2 M phosphate-citrate buffer at pH 7.8. U...
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[DNA Purification] Radioactive Proliferation Assay 日期：2005-07-18 00:00:00 点击：220 好评：0
Monday, November 24, 2003 Description The Proliferation Assay allows to determine the number of cells that are growing in the absence or presence of certain proliferation affecting agents, e.g. TNF-alpha or anti-Fas antibody (IPO-4). Proce...