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[DNA Purification] RNA EXTRACTION METHOD FOR COTTON 日期：2005-07-18 00:00:00 点击：153 好评：0
Description RNA EXTRACTION METHOD FOR COTTON Based on: Wan, C.-Y. and Wilkins, T.A. 1994. Anal. Bioch. 223:7-12. Procedure Collect young expanding leaves (or other tissue) and freeze in liquid N2 and store at -80°C. Pulverize tissue to a...
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[DNA Purification] CHLOROPLAST DNA ISOLATION 日期：2005-07-18 00:00:00 点击：169 好评：0
Friday, November 07, 2003 Description CHLOROPLAST DNA ISOLATION Procedure Grind in blender with 7 vol LL for maize, 10 for sorghum. Use MCE at 350 g/L, and if necessary, 5 ml 1 M DIECA/L. Cut leaves into ca. 1 strips prior to blending. Squ...
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[DNA Purification] NUCLEI ISOLATION 日期：2005-07-18 00:00:00 点击：141 好评：0
Friday, November 07, 2003 Description NUCLEI ISOLATION FROM PLANTS Procedure Harvest, weigh tissue, . Dump into N2 in ice bucket; break up with spatula/scissors (ca. 300 ml N2). Dump into Waring blender; cover with cheesecloth, grind at ma...
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[DNA Purification] ARABIDOPSIS THALIANA DNA ISOLATION PROCEDURE 日期：2005-07-18 00:00:00 点击：135 好评：0
Friday, November 07, 2003 Description Nuclei isolation modified from Hamilton, Kunsch, and Temperli (1972) Anal. Biochem. 49:48-57; Procedure Procedure: (all steps at 0-4C unless indicated) Harvest 100 g tissue (plants can be any age up to...
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[DNA Purification] DNA from plasma 日期：2005-07-18 00:00:00 点击：162 好评：0
Friday, October 24, 2003 Description Sometimes the only material available on a subject is stored plasma or serum samples. The DNA content in such samples is generally low, but sufficient to serve as template for PCR reactions. Many diseas...
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[DNA Purification] Detection of Recombinant Proteins by ELISA 日期：2005-07-18 00:00:00 点击：146 好评：0
Thursday, October 09, 2003 Description Copyright 1993, 1996, and 1997 by Mark Barton Frank, Ph.D. Proper citation for data acquired from this document is: McCubbin, V. and Frank, M. B. Detection of Recombinant Proteins by ELISA. In: Frank,...
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[DNA Microarray] DNA Microprep Isolation from Plants 日期：2005-07-18 00:00:00 点击：275 好评：0
Friday, November 21, 2003 Description The original maize DNA miniprep protocol is used extensively for many plant species and different tissues. This slightly modified version is acceptable for most DNA extractions. The procedure has the a...
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[DNA Microarray] DNA Miniprep Isolation from Plants 日期：2005-07-18 00:00:00 点击：272 好评：0
Description Procedure 1. Place 0.5 to 2 g (1 g is ideal) of plant tissue in a mortar and add an excess of liquid nitrogen. As the liquid nitrogen evaporates, grind the tissue thoroughly into a fine powder. 2. Transfer the plant powder into...
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[DNA Microarray] Plant Total DNA Isolation 日期：2005-07-18 00:00:00 点击：268 好评：0
Description This protocol describes a method for isolating DNA from plant tissue. Procedure 1. Preheat the CTAB Isolation Buffer at 60°C. 2. Grind 2 g of fresh, leaf tissue to a powder in Liquid Nitrogen in a chilled mortar and pestle. 3....
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[DNA Microarray] Cyanine Dye Purification Protocol 日期：2005-07-18 00:00:00 点击：266 好评：0
Friday, November 21, 2003 Description Cyanine Dye Purification Protocol Procedure Preparation of salt buffers The buffers are used for both HPLC purification and Sep-Pak purification. High salt buffer (HSB): 1M triethylammonium acetate, pH...