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[DNA Purification] Radioactive DNA Fragmentation Assay 日期：2005-07-18 00:00:00 点击：242 好评：0
Monday, November 24, 2003 Description The DNA Fragmentation Assay allows you to determine the amount of DNA that is degraded upon treatment of cells with certain agents, e.g. with TNF-alpha or anti-Fas antibody (IPO-4). The principle of th...
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[DNA Purification] Polysomal RNA isolation and purification 日期：2005-07-18 00:00:00 点击：240 好评：3
Friday, November 21, 2003 Description This protocol is optimized for isolation of polysomal RNA from leaves of mature Arabidopsis thaliana. Procedure 1. Cool rotor (Beckman SW55Ti) at 4C at least 1 hour prior to use. 2. Warm 20% detergent...
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[DNA Purification] PHAGE PURIFICATION WITH DE52 日期：2005-07-18 00:00:00 点击：152 好评：0
Friday, November 21, 2003 Description PHAGE PURIFICATION WITH DE52 Procedure 1. Grow fresh o/n of appropriatre cells (eg. KW251. 2. To 10ml LMM in a 100ml flask add 0.2ml o/n and a single plaque. 3. Let stand for 10min. then shake for 3-4h...
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[DNA Purification] Hoechst Fluorometric quantitation of DNA 日期：2005-07-18 00:00:00 点击：160 好评：0
Tuesday, November 18, 2003 Description The concentration of DNA in solutions containing contaminating protein or RNA can be determined quite accurately by measuring the increased fluorescence of the Hoechst dye 33258 upon intercalating int...
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[DNA Purification] Gene-Kleen protocol 日期：2005-07-18 00:00:00 点击：188 好评：0
Tuesday, November 18, 2003 Description Purification of DNA from agarose gels is an essential method involved in the sub-cloning of DNA fragments. The following method describes a variation of the method of Vogelstein and Gillespie, 1979 (P...
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[DNA Purification] De-phosphorylation of DNA 日期：2005-07-18 00:00:00 点击：191 好评：0
Tuesday, November 18, 2003 Description The preferred method of de-phosphorylation uses the buffer system described by Pharmacia for most of their restriction endonucleases. Procedure 1) After digestion of DNA with the appropriate restricti...
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[DNA Purification] nup4a-313 Shuttling in Yeast 日期：2005-07-18 00:00:00 点击：164 好评：0
Tuesday, November 18, 2003 Description nup4a-313 Shuttling in Yeast Procedure Method 1. Transform a plasmid containing YFG-GFP under the control of the galactose inducible promoter (pGAL1-10)a into a nup49-313 strainb 2. Grow the transform...
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[DNA Purification] Rapid Genomic Prep Yeast 日期：2005-07-18 00:00:00 点击：152 好评：0
Tuesday, November 18, 2003 Description Rapid Genomic Prep Procedure 1. Grow 5 ml yeast cultures to saturation. 2. Collect cells by centrifugation and resuspend in 0.5 ml of water. Transfer cells to 1.5 ml microfuge tube and collect by a 5...
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[DNA Purification] Genomic DNA Extraction - Quick Protocol 日期：2005-07-18 00:00:00 点击：156 好评：0
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[DNA Purification] Genomic DNA Extraction - CTAB Protocol 日期：2005-07-18 00:00:00 点击：248 好评：0
Monday, November 10, 2003 Description Genomic DNA Extraction - CTAB Protocol Procedure Place 3 medium-large Arabidopsis rosette leaves in an eppen tube Homogenise leaves in the eppendorf tube Add 500l of 2x CTAB/Mercaptoethanol buffer, kee...