IHC Washing Buffers

2006-01-22 14:42 bioguider 本站原创阅读 0

核心摘要： 免疫组化（IHC）中磷酸盐缓冲液（PB）作为洗涤缓冲液的配制方法：0.2M PB（Na₂HPO₄ 21.8g，NaH₂PO₄ 6.4g，蒸馏水1000ml）及0.1M PB（Na₂HPO₄ 10.9g，NaH₂PO₄ 3.2g，蒸馏水1000ml），均需调节pH至7.4并室温保存。该缓冲体系通过维持稳定的pH环境，确保抗原抗体反应的特异性与可重复性。

IHC Washing Buffers

Phosphate Buffer

Phosphate Buffer (0.2M PB, pH 7.4):

Na2HPO4 -------------------------------------- 21.8 g

NaH2PO4 -------------------------------------- 6.4 g

Distilled water --------------------------------- 1000 ml

Mix to dissolve and adjust pH to 7.4

Store this solution at room temperature.

Phosphate Buffer (0.1M PB, pH 7.4):

Na2HPO4 ------------------------------------- 10.9 g

NaH2PO4 ------------------------------------- 3.2 g

Distilled water -------------------------------- 1000 ml

Mix to dissolve and adjust pH to 7.4

Store this solution at room temperature.

TAGS: 磷酸盐缓冲液 pH值洗涤缓冲液免疫组化

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