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[Cell Culture] Cloning cells by limiting dilution 日期：2006-07-24 14:27:26 点击：333 好评：0
Monday, June 07, 2004 Description This protocol describes a method that can be used to clone individual cells. This is done by limiting dilution and examination of plates for single cells. Growth factors or selection components may be adde...
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[Cell Culture] PAH XmnI Polymorphism 日期：2006-07-24 14:27:24 点击：248 好评：0
Saturday, May 15, 2004 Description PAH XmnI Polymorphism Procedure XmnI; LOCATED ABOUT 2 KB 3' OF THE MSPI POLYMORPHISM IN INTRON 8 Template: Genomic DNA 100-200 ng/reaction volume of 25 l Primer 1. PAHXMN1 5 -CTG TAC TTG TAA GAT GCA GC-3'...
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[Cell Culture] APOB XbaI Polymorphism 日期：2006-07-24 14:27:24 点击：227 好评：0
Saturday, May 15, 2004 Description APOB XbaI Polymorphism Procedure Xba I Template: Genomic DNA 100-200 ng/reaction volume of 25 l Primer 1. APOBXB1: 5'-AAA TAA CCT TAA TCA TCA ATT GGT 3' 100ng/reaction Primer 2. APOBXB2: 5'-GGT TCT TAG CA...
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[Cell Culture] COMT BglI Polymorphism 日期：2006-07-24 14:27:24 点击：304 好评：0
Saturday, May 15, 2004 Description COMT BglI Polymorphism Procedure Bgl I (modified from Chen et al., 1996) Template: Genomic DNA 100-200 ng/reaction volume of 25 l. Primer 1. COMTBGL1F 5'--TGC GGA AGG GGA CAG TGC TAC--3' 100 ng/Reaction P...
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[Cell Culture] PAH BglII Polymorphism 日期：2006-07-24 14:27:23 点击：273 好评：0
Saturday, May 15, 2004 Description PAH BglII Polymorphism Procedure BglII; LOCATED 5' OF EXON 1 Template: Genomic DNA 100-200 ng/reaction volume of 25 l Primer 1. B2PAH1 5' -GCA GGA AAC TCT CTG ACT TTG G -3' 100 ng/reaction Primer 2. B2PAH...
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[Cell Culture] RET TaqI Polymorphism 日期：2006-07-24 14:27:23 点击：219 好评：0
Saturday, May 15, 2004 Description RET TaqI Polymorphism Procedure Taq I polymorphism located in Exon 13 Template: Genomic DNA 50-100 ng/reaction volume of 25 ul Primer 1. RETEX13F: 5'-CTC TCT GTC TGA ACT TGG GC-3' 60 ng/Reaction Primer 2....
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[Cell Culture] PAH MspI Polymorphism 日期：2006-07-24 14:27:22 点击：253 好评：0
Saturday, May 15, 2004 Description PAH MspI Polymorphism Procedure MspI; LOCATED NEAR THE 5' END OF INTRON 8 Template: Genomic DNA 100-200 ng/reaction volume of 25 l Primer 1. PAHM 5 -GAA GCA TAT TGT ATC TGC CC -3' 100 ng/reaction Primer 2...
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[Cell Culture] HOXB3 TaqI Polymorphism 日期：2006-07-24 14:27:22 点击：226 好评：0
Saturday, May 15, 2004 Description HOXB3 TaqI Polymorphism Procedure TaqI in HOXB3; Clone pH 2-1-5 Template: Genomic DNA 100-200 ng/reaction volume of 25 l Primer 1. H2GCK3 '5-TAT TTT TGT GAT CAA TGG AGA CC 100 ng/reaction Primer 2. H2GCK3...
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[Cell Culture] ADH2 Intron 3 RsaI Polymorphism 日期：2006-07-24 14:27:21 点击：193 好评：0
Saturday, May 15, 2004 Description ADH2 Intron 3 RsaI Polymorphism Procedure TEMPLATE: Genomic DNA 100ng/reaction volume of 25 l PRIMER 1. A2IN3DW3 5' ATA TTT ATT TTA CCC TAA ACT TAT G 3' PRIMER 2. A2IN3UP2 5' GAG CTA AAA CAT ACT TTG GAT A...
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[Cell Culture] Metaphase chromosome preparation 日期：2006-07-24 14:27:21 点击：233 好评：2
Saturday, May 15, 2004 Description Metaphase chromosome preparation Procedure Incubate culture for 72 hours in CO2 cell culture incubator, mix flask 1-2 times per day Add Colcemid (about 45 min before harvesting) Make 2 aliquots and transf...