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[Cell Culture] PDS1::HA IMMUNOFLUORESCENCE 日期：2006-07-24 14:27:15 点击：130 好评：0
Monday, May 10, 2004 Description PDS1::HA IMMUNOFLUORESCENCE Procedure Cultures: 1500, 1501 Fixations: 10, 20, 30 and 45 minutes in 4% formaldehyde, 0.1 M KPO4 pH 6.5; 15 minutes in 4% formaldehyde, 50 mM KPO4 pH 6.5, 0.5 mM MgCl2 Grow cel...
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[Cell Culture] IIF Protocol (modifications for maintaining GFP fluorescence 日期：2006-07-24 14:27:14 点击：132 好评：0
Monday, May 10, 2004 Description IIF Protocol (modifications for maintaining GFP fluorescence Procedure Grow cells to OD 0.5 Harvest 0.5-1 ml cells. Resuspend in PBS, and add PFA to final concentration of 4%. If using the regular formaldeh...
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[Cell Culture] Iindirect immunoflourescence for yeast: 日期：2006-07-24 14:27:14 点击：154 好评：0
Monday, May 10, 2004 Description Iindirect immunoflourescence for yeast: Procedure 1mL yeast (~0.2-0.5 OD) + 0.1mL 36% formaldehyde, 60'/Rt Wash 2x 1mL H2O (spin 10K, 5). Wash in 1mL SK and resuspend in 450 ul SK. Sonicate 5 sec. lowest se...
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[Cell Culture] Immunofluorescence Yeast 日期：2006-07-24 14:27:13 点击：139 好评：0
Wednesday, May 05, 2004 Description Immunofluorescence Yeast Procedure 1) Grow cells in YEPD or defined media to a concentration of 1-5 X107(protocol is for ~5 ml of exponentially growing cells). 2) Add 0.6 ml of 37% formaldehyde solution...
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[Cell Culture] Yeast FACS 日期：2006-07-24 14:27:12 点击：157 好评：0
Wednesday, May 05, 2004 Description Yeast FACS Procedure 1. Grow cells to less than OD = 1 (i.e 2. Spin down approx 500 ul. Amount of cells should be about 5 to 10 ul volume. Too many cells does not work! 3. Resuspend in 300 l and dropwise...
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[Cell Culture] Setting up 2 or 3 Colour FACS analysis 日期：2006-07-24 14:27:11 点击：97 好评：0
Wednesday, May 05, 2004 Description These detailed instructions are for the FACScans with FACSMates, but the principles also apply to setting up 3 (or even 4) colour analysis on the FACSort. Procedure 1) It is important to set the colour c...
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[Cell Culture] NEOPTERIN ENZYME IMMUNOASSAY (EIA) TEST PROCEDURE 日期：2006-07-24 14:27:10 点击：147 好评：0
Wednesday, May 05, 2004 Description Neopterin [D-erythro-neopterin] belongs to the group of pteridines. Neopterin as well as other pteridines are derived in vivo from guanosinetriphophate (GTP). The enzyme GTP-cyclohydrolase-I catalyses th...
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[Cell Culture] Sandwich ELISA for SCCA1 and SCCA2 日期：2006-07-24 14:27:09 点击：135 好评：0
Wednesday, May 05, 2004 Description Sandwich ELISA for SCCA1 and SCCA2 Procedure 1. Dilute affinity purified rabbit anti-SCCA 1:400 in PBS (appropriate dilution of this antibody needs to be determined following each batch affinity purifica...
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[Cell Culture] ELISA, Antibody capture 日期：2006-07-24 14:27:09 点击：149 好评：0
Wednesday, May 05, 2004 Description ELISA, Antibody capture Procedure 1. Dilute protein in PBS (2.5-5 mg/ml), add 100 ml/well. Incubate overnight at 4 C or at room temperature for 1 h. 2. Block plates with 250 ml PBS/1% BSA for overnight a...
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[Cell Culture] DETECTION OF ISOTYPE SPECIFIC IgG ANTIBODIES 日期：2006-07-24 14:27:08 点击：154 好评：0
Wednesday, May 05, 2004 Description Response to immunization with Tetanus Toxoid Vaccine is an indicator of immune function in infants and children which can be measured by comparing the amount of specific antibody detected before and afte...