Thursday, October 16, 2003 Description A method that worked for me, pretty close to the commercial description Procedure 1 - Preparation of cells for transfection Day 1: Plate the cells one-day before the transfection experiment. The appro...
Thursday, October 16, 2003 Description Protocol for large scale Plasmid DNA isolation Procedure Y To be read as micro (mu) 1.5ml of LB medium containing 50Yg/Yl ampicillin [Sigma A-0166] was innoculated with a single bacterial colony. The...
Wednesday, October 08, 2003 Description For preparation of competent bacterial cells (14), a glycerol cell culture stock of the respective E. coli strain is thawed and added to 50 ml of liquid media. This culture then is preincubated at 37...
Wednesday, October 08, 2003 Description DNA ligations are performed by incubating DNA fragments with appropriately linearized cloning vector in the presence of buffer, rATP, and T4 DNA ligase (1,2). For random shotgun cloning, sonicated or...
Saturday, May 15, 2004 Description NGFR TaqI(a) Polymorphism Procedure TaqI(a) (7.18 KB DWST OF EXON 6) Template: Genomic DNA 100-200 ng/reaction Primer 1. NGFRTQAF1 5' GAC ACT GGT TAT ACT CTA GTC C 3' 100 ng/reaction Primer 2. NGFRTQAR1 5...
Description DRD2 TaqI "A" Polymorphism Procedure Taq I "A" Located 10 kb downstream from exon 8 TEMPLATE: Genomic DNA 100-200 ng/reaction volume of 25 l. Primer 1: 5014 MOD: '5- CCT TCC TGA GTG TCA TCA AC 100 ng/reaction Primer 2: TAQACK1:...
Saturday, May 15, 2004 Description RBP3 BglII Polymorphism Procedure Bgl II polymorphism located near 5' end of intron 2 Genomic DNA: 100-200 ng/reaction volume of 25 ul Primer 1. RBP3BGL1: 5'-ACC CTG TGA CAC AGC AGA GG-3' 100 ng/Reaction...
Description DRD2 TaqI "B" Polymorphismq Procedure Taq I "B" Bcl I Located in intron 1, approximately 1 kb upstream from exon 2 (The Taq I "B" and the Bcl I sites are 12 bp apart) Template: Genomic DNA 100-200 ng/ reaction volume of 25 l. P...
Saturday, May 15, 2004 Description RBP3 MspI Polymorphism Procedure MspI Polymorphism located near 3' end of intron 1 Template: Genomic DNA 100-200 ng/reaction volume of 25 ul Primer 1. RBP3MSP3: 5'- CTG TAA GAG TTG GAG GT-3' 100 ng/Rx Pri...
Saturday, May 15, 2004 Description D10S94 PvuII Polymorphism Procedure Pvu II Template: Genomic DNA 100-200 ng/reaction volume of 25 l Primer 1. S94PVUA 5 -AGCTCACCCAGATCCTAA -3' 90 ng/reaction Primer 2. S94PVUB 5' -CCTCCCTTCAGTAGACAC -3'...