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[DNA Sequencing] ACADM TaqI Polymorphism 日期：2005-07-18 00:00:00 点击：274 好评：0
Saturday, May 15, 2004 Description ACADM TaqI Polymorphism Procedure TaqI site (located in intron 7) Template: Genomic DNA 100-200 ng/reaction volume of 25 l Primer 1. ACADMTAQI: 5'-GAT CCA GTG ACT CAG AAG-3' 100 ng/reaction Primer 2. ACAD...
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[DNA Sequencing] DM1 Alu Polymorphism 日期：2005-07-18 00:00:00 点击：284 好评：0
Saturday, May 15, 2004 Description DM1 Alu Polymorphism Procedure Template: Genomic DNA 100-200 ng/reaction volume of 25 ul Primer 1: 405: 5'-CTG TAT ACT CAG CTA CTA GGG T-3' 50 ng/rx Primer 2: 491: 5'-AAA TAG GCT GGA CCG CGG-3' 50 ng/rx P...
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[DNA Sequencing] For Double-stranded reactions: Sequenase[TM] catalyzed seque 日期：2005-07-18 00:00:00 点击：273 好评：0
Thursday, November 20, 2003 Description For Double-stranded reactions Procedure 1. Add the following to a 1.5 ml microcentrifuge tube: 5 ulds DNA (5 ug) 4 ul1 N NaOH 3 ulddH2O 12 ul 2. Incubate the reaction at 65-70degC for 5 minutes, and...
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[DNA Sequencing] Taq-polymerase catalyzed cycle sequencing using fluorescent- 日期：2005-07-18 00:00:00 点击：192 好评：0
Thursday, November 20, 2003 Description One of the major problems in DNA cycle sequencing is that when fluorescent primers are used the reaction conditions are such that the nested fragment set distribution is highly dependent upon the tem...
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[DNA Sequencing] Taq-polymerase catalyzed cycle sequencing using fluorescent- 日期：2005-07-18 00:00:00 点击：277 好评：0
Thursday, November 20, 2003 Description Each base-specific fluorescent-labeled cycle sequencing reaction routinely included approximately 100 or 200 ng Biomek isolated single-stranded DNA for A and C or G and T reactions, respectively. Dou...
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[DNA Sequencing] Radiolabeled sequencing gel preparation, loading, and electr 日期：2005-07-18 00:00:00 点击：188 好评：0
Thursday, November 20, 2003 Description To prepare polyacrylamide gels for DNA sequencing, the appropriate amount of urea is dissolved by heating in water and electrophoresis buffer, the respective amount of deionized acrylamide-bisacrylam...
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[DNA Sequencing] Bst-catalyzed radiolabeled DNA sequencing For single-strande 日期：2005-07-18 00:00:00 点击：181 好评：0
Thursday, November 20, 2003 Description Bst DNA polymerase-catalyzed radiolabeled two-step sequencing reactions For single-stranded DNA sequencing Procedure 1. Prepare the following extension reaction in a microcentrifuge tube: 750 ngM13 t...
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[DNA Sequencing] DNA sequencing gels 日期：2005-07-18 00:00:00 点击：225 好评：0
Tuesday, November 18, 2003 Description sequencing gels Procedure 1) Clean the glass plates extensively with detergent and water, tap water, distilled water and finally ethanol. Wipe dry with a clean paper towel. 2) Siliconize the smaller o...
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[DNA Sequencing] DNA Sequencing Sequencing reaction 日期：2005-07-18 00:00:00 点击：246 好评：0
Tuesday, November 18, 2003 Description Sequencing reaction Procedure 1) Denature dsDNA by the addition of 8ul DNA (approximately 3ug) to a sterile microcentrifuge tube containing 2ul freshly made 2M NaOH vortexed briefly and incubate at ro...
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[DNA Sequencing] Measurement of DNA concentration using PanVera fluorescence 日期：2005-07-18 00:00:00 点击：196 好评：0
Tuesday, November 18, 2003 Description Measurement of DNA concentration using PanVera fluorescence assay Procedure This Dye measures DNA concentration using a fluorescent dye which binds only double stranded DNA. The fluorescence of this d...